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Chin. Phys. B, 2022, Vol. 31(4): 044304    DOI: 10.1088/1674-1056/ac4a6f

Non-invasive and low-artifact in vivo brain imaging by using a scanning acoustic-photoacoustic dual mode microscopy

Wentian Chen(陈文天)1, Chao Tao(陶超)1,†, Zizhong Hu(胡仔仲)2, Songtao Yuan(袁松涛)2,‡, Qinghuai Liu(刘庆淮)2, and Xiaojun Liu(刘晓峻)1,§
1 Ministry-of-Education Key Laboratory of Modern Acoustics, Department of Physics, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China;
2 Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medial University, Nanjing 210029, China
Abstract  Photoacoustic imaging is a potential candidate for in vivo brain imaging, whereas, its imaging performance could be degraded by inhomogeneous multi-layered media, consisted of scalp and skull. In this work, we propose a low-artifact photoacoustic microscopy (LAPAM) scheme, which combines conventional acoustic-resolution photoacoustic microscopy with scanning acoustic microscopy to suppress the reflection artifacts induced by multi-layers. Based on similar propagation characteristics of photoacoustic signals and ultrasonic echoes, the ultrasonic echoes can be employed as the filters to suppress the reflection artifacts to obtain low-artifact photoacoustic images. Phantom experiment is used to validate the effectiveness of this method. Furthermore, LAPAM is applied for in-vivo imaging mouse brain without removing the scalp and the skull. Experimental results show that the proposed method successfully achieves the low-artifact brain image, which demonstrates the practical applicability of LAPAM. This work might improve the photoacoustic imaging quality in many biomedical applications which involve tissues with complex acoustic properties, such as brain imaging through scalp and skull.
Keywords:  photoacoustic microscopy      scanning acoustic microscopy      noninvasive      low-artifact      brain imaging  
Received:  20 August 2021      Revised:  25 November 2021      Accepted manuscript online:  12 January 2022
PACS:  43.35.Ud (Thermoacoustics, high temperature acoustics, photoacoustic effect)  
  43.35.Sx (Acoustooptical effects, optoacoustics, acoustical visualization, Acoustical microscopy, and acoustical holography)  
  78.20.Pa (Photoacoustic effects)  
  81.70.Cv (Nondestructive testing: ultrasonic testing, photoacoustic testing)  
Fund: Project supported by the National Natural Science Foundation of China (Grant Nos. 12027808, 11874217, 11834008, 81900875, and 81770973) and Natural Science Foundation of Jiangsu Province, China (Grant No. BK 20181077).
Corresponding Authors:  Chao Tao, Songtao Yuan, Xiaojun Liu     E-mail:;;

Cite this article: 

Wentian Chen(陈文天), Chao Tao(陶超), Zizhong Hu(胡仔仲), Songtao Yuan(袁松涛), Qinghuai Liu(刘庆淮), and Xiaojun Liu(刘晓峻) Non-invasive and low-artifact in vivo brain imaging by using a scanning acoustic-photoacoustic dual mode microscopy 2022 Chin. Phys. B 31 044304

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