| SPECIAL TOPIC—8th IUPAP International Conference on Biological Physics |
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Catch-bond behavior of DNA condensate under tension |
| Li Wei (李伟)a, Wong Wei-Juanb, Lim Ci-Jib, Ju Hai-Peng (车海鹏)a, Li Ming (李明)a, Yan Jie (严洁)b, Wang Peng-Ye (王鹏业)a |
a Beijing National Laboratory for Condensed Matter Physics and Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China;
b Department of Physics, National University of Singapore, Singapore 117542, and Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore |
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Abstract Toroid formation is an important mechanism underlying DNA condensation, which has been investigated extensively by single-molecule experiments in vitro. Here, the de-condensation dynamics of DNA condensates were studied using magnetic tweezers combined with Brownian dynamics simulations. The experimental results revealed a surprising non-monotonic dependence of the unfolding rate on the force applied under strong adhesion conditions, resembling the catch-bond behavior reported in the field of ligand-receptor interactions. Simulation results showed that the different unfolding pathways of DNA condensate under large forces derive from the force-dependent deformation of the DNA toroid, which explains the catch-bond behavior of DNA condensate in the magnetic tweezers experiments. These results challenge the universality of the regular toroidal DNA unwrapping mechanism and provide the most complete description to date of multivalent cation-dependent DNA unwrapping under tension.
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Received: 09 April 2015
Revised: 13 July 2015
Accepted manuscript online:
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PACS:
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87.15.H-
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(Dynamics of biomolecules)
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82.37.Rs
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(Single molecule manipulation of proteins and other biological molecules)
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87.15.La
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(Mechanical properties)
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87.16.Sr
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(Chromosomes, histones)
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| Fund: Project supported by the National Natural Science Foundation of China (Grant Nos. 11104341, 11474346, 11274374, and 61275192), the National Key Basic Research Program of China (Grant No. 2013CB837200), and the Mechanobiology Institute at National University of Singapore. These authors are contributed equally to this work. |
Corresponding Authors:
Li Wei, Yan Jie, Wang Peng-Ye
E-mail: weili007@iphy.ac.cn;phyyj@nus.edu.sg;pywang@iphy.ac.cn
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Cite this article:
Li Wei (李伟), Wong Wei-Juan, Lim Ci-Ji, Ju Hai-Peng (车海鹏), Li Ming (李明), Yan Jie (严洁), Wang Peng-Ye (王鹏业) Catch-bond behavior of DNA condensate under tension 2015 Chin. Phys. B 24 128704
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